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KMID : 0377619740270060553
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Korean Jungang Medical Journal
1974 Volume.27 No. 6 p.553 ~ p.558
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Immunogical Determination of Luteinizing Hormone In the Urine and Serum of Contraceptive Users
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Abstract
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Blood serum and urine levels of luteinizing hormone (L.H.) in oral contraceptive (Ovulen; ethynodiol diacetate 1mg-; mestranol 100 mcg) users were measured using hemagglutination inhibition reaction based upon the cross reaction between the HCG-antiserum to L.H.
Following results were obtained;
1) Human Chorionic Gonadotropin (H.C.G.) of relatively high purity (5000 IU/ mg) have been used as antigen for the immunization of rabbits and high titer of antiserum was obtained through the effects of Freund´s adjuvant and booster injections.
2) Gel diffusion method of Ouchterlony revealed only one clear precipitation line between the H.C.G. and its antiserum, indicating that the used antigen was relatively pure and the antisera contained negligible amount of nonspecific antibody.
3) The sensitivity of hemagglutination reaction in this study was about 50 IU/L.
4) One volume of urine or blood serum was treated with four volumes of ace-tone and L.H. containing fraction was washed with one volume of absolute alcohol and ether, then dissolved in 1/10 volume of pH 6. 4 phosphate buffered saline, thus concentrating the original specimen 10 times.
5) Three out of seven healthy parous women selected in this study started Ovulen from 5th MCD (menstrual cycle day).
6) Early morning samples of the urine and blood serum were collected from 10 th MCD showed no L.H. peak after Ovulen administration.
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KEYWORD
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